| Ge Qianqian,Li Zhengdao,Li Jitao,Wang Jiajia,Li Jian.Effects of acute salinity stress on the survival and prophenoloxidase system of Exopalaemon carinicauda[J].Acta Oceanologica Sinica,2020,39(4):57-64 |
| 急性盐度胁迫对脊尾白虾酚氧化酶系统的影响 |
| Effects of acute salinity stress on the survival and prophenoloxidase system of Exopalaemon carinicauda |
| 投稿时间：2018-01-08 |
| DOI：10.1007/s13131-020-1582-4 |
| 中文关键词: 脊尾白虾 酚氧化酶系统 盐度胁迫 免疫 耐受范围 |
| 英文关键词:Exopalaemon carinicauda prophenoloxidase system salinity stress immunity tolerant range |
| 基金项目:The National Key R&D Program of China under contract No. 2019YFD0900400; the China Agriculture Research System under contract No. CARS-48; the National Natural Science Foundation of China under contract No. 31702319; the Program of Shandong Leading Talent under contract No. LNJY2015002; the Central Public-interest Scientific Institution Basal Research Fund, CAFS under contract No. 2019ZD0603. |
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| 中文摘要: |
| 脊尾白虾是一种生活在河口及沿海水域的广盐性虾类。本研究检测比较了急性盐度胁迫下脊尾白虾幼虾和成虾两个不同发育时期存活率、酚氧化酶系统两个相关基因(EcLGBP, EcproPO)转录水平及酚氧化酶活力。结果显示脊尾白虾幼虾对盐度的耐受范围比成虾广。幼虾的EcLGBP and EcProPO 基因在低盐胁迫下上调表达，且在20-40 盐度范围内无显著变化，而PO活力在低盐胁迫下高于高盐胁迫。成虾EcLGBP and EcproPO基因在盐度胁迫后表达量出现不同程度的上调，且在30-40盐度范围内基因表达及PO活力变化不大。以上表明盐度20-40及30-40可能分别是脊尾白虾幼虾和成虾免疫耐受范围。 |
| 英文摘要: |
| The ridgetail white prawn Exopalaemon carinicauda is a euryhaline shrimp species in the estuarine and coastal areas of China. In this study, survival rates, transcription levels of two prophenoloxidase system-related genes (EcLGBP and EcproPO) and PO activity were determined quantitatively in juvenile and adult E. carinicauda under different salinity levels. The results showed that E. carinicauda juveniles could survive in a wider range of salinity conditions than adults. For juvenile E. carinicauda, the expression levels of EcLGBP and EcProPO were up-regulated in low salinities and showed no significant difference at 20–40, while PO activities in low salinities were higher compared to those in high salinities. For adult E. carinicauda, the expression profiles of EcLGBP and EcproPO had a different trend of up-regulation in salinity stress treatments and no obvious difference was observed in the gene expression levels and PO activity between 30 and 40. The salinity tolerance range of immunity for juvenile and adult E. carinicauda is 20–40 and 30–40, respectively. |
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