| WANG Jian,WANG Zhaohui,ZHAO Jiangang.Isolation, characterization and implications of the bacterial communities associated with established cultures of Chattonella marina (Raphidophyceae) and Skeletonema costatum (Bacillariophyceae)[J].Acta Oceanologica Sinica,2019,38(1):128-135 |
| 针胞藻海洋卡盾藻(Chattonella marina)和硅藻中肋骨条藻(Skeletonema costatum)藻际细菌的分离、鉴定及影响研究 |
| Isolation, characterization and implications of the bacterial communities associated with established cultures of Chattonella marina (Raphidophyceae) and Skeletonema costatum (Bacillariophyceae) |
| 投稿时间：2017-11-22 |
| DOI：10.1007/s13131-019-1379-5 |
| 中文关键词: 藻际环境 海洋细菌 海洋卡盾藻 中肋骨条藻 旋链角毛藻 生长 |
| 英文关键词:phycosphere marine bacteria Chattonella marina Skeletonema costatum Chaetoceros curvisetus algal growth |
| 基金项目:The National Natural Science Foundation of China under contract Nos 41476132 and 41276154. |
| 作者 | 单位 | E-mail | | 王剑 | 暨南大学生命科学技术学院, 水体富营养化与赤潮防治广东普通高校重点实验室, 广州, 510632
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China | | | 王朝晖 | 暨南大学生命科学技术学院, 水体富营养化与赤潮防治广东普通高校重点实验室, 广州, 510632
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China | twzh@jnu.edu.cn | | 赵建刚 | 暨南大学生命科学技术学院, 水体富营养化与赤潮防治广东普通高校重点实验室, 广州, 510632
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, China | |
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| 中文摘要: |
| 对实验室培养的两株海洋微藻海洋卡盾藻和中肋骨条藻的可培养细菌进行了分离鉴定，分别在海洋卡盾藻和硅藻中肋骨条藻不同生长时期分离得到48株和34株可培养细菌，去除重复序列后得到12株不同的菌株。这些菌株分属α-变形杆菌纲、γ-变形杆菌纲、拟杆菌和放线菌。α-变形杆菌纲在两种藻的藻际环境中占据优势，其中红杆菌科是最为常见的科，中肋骨条藻的藻际细菌比海洋卡盾藻更为多样化。海洋卡盾藻生长后期细菌密度大幅度上升，可能导致了藻细胞的衰亡。中肋骨条藻相关细菌中有一株对旋链角毛藻的生长具有明显抑制作用，而大部分海洋卡盾藻相关细菌对角毛藻的生长具有抑制作用。本研究结果说明海洋卡盾藻的藻际细菌也许对其与硅藻的种间竞争中具有一定作用。 |
| 英文摘要: |
| Cultivable bacteria coexisting in the cultures of two microalgal species, Chattonella marina (Raphidophyceae) and Skeletonema costatum (diatom, Bacillariophyceae), which have been maintained in the laboratory for several years, were examined in this study. Forty-eight and thirty-four cultivable bacterial strains were isolated from different growth stages of C. marina and S. costatum cultures, respectively. A total of twelve unique bacterial phylotypes were isolated. These bacterial phylotypes belonged to Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes, and Actinobacteria. Alphaproteobacteria predominated in phycospheres of both microalgae, and Rhodobacteraceae was the most common family. Bacterial phylotypes were more diversified in cultures of S. costatum than in those of C. marina. Bacterial concentrations increased remarkably after the late stationary phase of C. marina, which might account for the decline in algal cells. One phylotype of S. costatum-associated bacteria had inhibitory effects on Chaetoceros curvisetus (Bacillariophyceae). However, most bacterial phylotypes from cultures of C. marina showed significant inhibition of the growth of C. curvisetus. The results suggested that bacteria associated with C. marina might have some ecological roles in its competition with diatoms. |
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