| SHI Feng,WEI Xiaoxue,FENG Jianfeng,SUN Yingxue,ZHU Lin.Variation of bacterial community associated with Phaeodactylum tricornutum in response to different inorganic nitrogen concentrations[J].Acta Oceanologica Sinica,2018,37(12):118-128 |
| 不同无机氮浓度下三角褐指藻藻际细菌群落变化研究 |
| Variation of bacterial community associated with Phaeodactylum tricornutum in response to different inorganic nitrogen concentrations |
| 投稿时间：2018-05-07 |
| DOI：10.1007/s13131-018-1272-7 |
| 中文关键词: 三角褐指藻 氮浓度 氮形态 细菌多样性 群落结构 γ-变形菌 |
| 英文关键词:Phaeodactylum tricornutum nitrogen concentrations nitrogen forms bacterial diversity community structure Gammaproteobacteria |
| 基金项目:The National Natural Science Foundation of China (NSFC) under contract No. 31470536; the National Key Research and Development Program of China under contract No. 2018YFC1406403. |
| 作者 | 单位 | E-mail | | 石峰 | 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300350 | | | 魏晓雪 | 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300350 | | | 冯剑丰 | 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300350 | fengjf@nankai.edu.cn | | 孙迎雪 | 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300350 | | | 朱琳 | 南开大学环境科学与工程学院, 污染过程与环境基准教育部重点试验室, 天津市城市生态环境修复与污染防治重点试验室, 天津 300350 | zhulin@nankai.edu.cn |
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| 中文摘要: |
| 藻际环境细菌和微藻间存在着独特的生态关系，它们可以通过转化和交换浮游植物分泌的有机质来影响其生理和代谢。尽管一些功能微生物在藻际环境的营养循环中具有重要作用，例如氮代谢，但是关于细菌群落与共生藻类如何响应环境中氮营养条件的改变目前并不十分清楚，其对于我们了解全球营养物质循环、藻华的形成以及生态系统功能至关重要。本文基于16S rRNA基因的高通量测序技术，分析了不同形态、不同浓度无机氮培养条件下三角褐指藻藻际环境中相关细菌群落的多样性和群落结构。系统发育分析结果表明，变形菌（Proteobacteria）和拟杆菌（Bacteroidetes）是所有样品中的优势菌，占序列总数的99.5%。同时发现，不同氮浓度培养条件下，细菌群落结构随微藻丰度的变化而改变。氮浓度及形态的变化对菌群结构的影响不显著。此外，γ-变形菌纲中三种细菌（Marinobacter；Algiphilus；Methylophaga）的相对丰度在氮限制培养条件下明显增加，它们可能在共生体系的氮转化过程中具有重要作用。 |
| 英文摘要: |
| Specific bacterial communities interact with phytoplankton in laboratory algal cultures. These communities influence phytoplankton physiology and metabolism by transforming and exchanging phytoplankton-derived organic matter. Functional bacterial groups may participate in various critical nutrients fluxes within these associations, including nitrogen (N) metabolism. However, it is unclear how bacterial communities and the associated algae respond to changes of phycosphere N conditions. This response may have far-reaching implications for global nutrient cycling, algal bloom formation, and ecosystem function. Here, we identified changes in the bacterial communities associated with Phaeodactylum tricornutum when co-cultured with different forms and concentrations of N based on the Illumina HiSeq sequencing of 16S rRNA amplicons. Phylogenetic analysis identified Proteobacteria and Bacteroidetes as the dominant phyla, accounting for 99.5% of all sequences. Importantly, bacterial abundance and community structure were more affected by algal abundance than by the form or concentration of inorganic N. The relative abundance of three gammaproteobacterial genera (Marinobacter, Algiphilus and Methylophaga) markedly increased in N-deficient cultures. Thus, some bacterial groups may play a role in the regulation of N metabolism when co-cultured with P. tricornutum. |
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