| OU Danyun,CHEN Bin,HADI Tri Aryono,SUHARSONO null,NIU Wentao,ALFIANSAH Yustian Rovi.Next-generation sequencing revealed specific microbial symbionts in Porites lutea with pigment abnormalities in North Sulawesi, Indonesia[J].Acta Oceanologica Sinica,2018,37(12):78-84 |
| 高通量测序技术揭示印度尼西亚北苏拉威西岛一株色素异常澄黄滨珊瑚特异的共生微生物群落 |
| Next-generation sequencing revealed specific microbial symbionts in Porites lutea with pigment abnormalities in North Sulawesi, Indonesia |
| 投稿时间：2017-06-15 |
| DOI：10.1007/s13131-018-1291-4 |
| 中文关键词: 澄黄滨珊瑚 色素异常 高通量测序 16s rRNA 细菌多样性 |
| 英文关键词:Porites lutea pigment abnormalities next-generation sequencing 16S rRNA bacterial diversity |
| 基金项目:The China-Indonesia Maritime Cooperation Fund Project "China-Indonesia Bitung Ecological Station Establishment"; the National Natural Science Foundation of China under contract No. 41506180; the Public Science and Technology Research Funds Projects of Ocean under contract No. 201505009. |
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| 中文摘要: |
| 细菌性疾病对珊瑚的健康影响造成了很大影响，而致病菌与珊瑚疾病之间的对应性还不明朗。色素异常现象是野外澄黄滨珊瑚一种常见的不健康状态。我们采用Illumina 16s rRNA基因高通量测序技术比较分析了健康和色素异常澄黄滨珊瑚共生细菌的群落组成差异。色素异常澄黄滨珊瑚共生细菌的多样性明显高于健康澄黄滨珊瑚，同时两种珊瑚的共生细菌群落组成也有很大差异。一些特定的细菌类群在澄黄滨珊瑚共生细菌中的比例很高，推测这些细菌种类可能是导致澄黄滨珊瑚色素异常的“罪魁祸首”。 |
| 英文摘要: |
| Bacterial diseases affecting corals pose an enormous threat to the health of coral reefs. The relationship between certain bacterial species and coral diseases remain largely unknown. Pigment abnormalities are common in Porites lutea. Here we used Illumina 16S rRNA gene sequencing to analyze the bacterial communities associated with healthy P. lutea and P. lutea with pigment abnormalities. We observed an increase of alpha diversity of the bacterial community of P. lutea with pigment abnormalities, relative to healthy corals. We then identified changes in the abundance of individual operational taxonomic units (OTUs) between pigmented and healthy corals. We were able to identify eight OTUs associated with pigment abnormalities, which are possibly the causative agents of pigment abnormalities. |
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