| ZHANG Xiaodong,YANG Shuang,SUN Jun,WU Chao,WANG Jing,ZHANG Guicheng,DING Changling.Morphology, ultrastructure and phylogeny of Cyanothece sp. (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean[J].Acta Oceanologica Sinica,2018,37(10):4-10 |
| 东印度洋分离的一株蓝杆藻的形态学,超显微结构以及系统发生分析 |
| Morphology, ultrastructure and phylogeny of Cyanothece sp. (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean |
| 投稿时间：2017-09-19 |
| DOI：10.1007/s13131-018-1297-y |
| 中文关键词: 蓝杆藻 蓝藻 形态学 16S rRNA nifH基因 印度洋 |
| 英文关键词:Cyanothece cyanobacteria morphology 16S rRNA gene nifH gene Indian Ocean |
| 基金项目:The National Natural Science Foundation of China under contract Nos 41876134, 41676112, 41276124, 41406155 and 41506182; the Natural Science Foundation of Tianjin under contract No. 17JCZDJC40000; the Exploration Program of Ocean with Science and Technology of Tianjin under contract No. KJXH2013-22; the Changjiang Scholar Program of Chinese Ministry of Education of China to Jun Sun. |
| 作者 | 单位 | E-mail | | 张晓东 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | | | 杨双 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | | | 孙军 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | phytoplankton@163.com | | 吴超 | 山东大学海洋研究院, 济南, 250110 | | | 王静 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | | | 张桂成 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | | | 丁昌玲 | 天津市海洋化学与资源重点实验室, 天津, 300457
天津科技大学海洋与环境学院, 天津, 300457 | |
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| 中文摘要: |
| 我们在印度洋赤道区域成功分离一株胶质包裹的绿色单细胞藻。结合显微镜观察，超显微结构分析以及分子生物学鉴定，我们将其鉴定为蓝藻门中具有很重要生态学意义的蓝杆藻。这株藻为椭圆形或棍形的单细胞，直径相对较小，平均为2.5到6.5μm。细胞超显微结构较为简单，类囊体贴壁排列，内部有窝状结构。细胞内有许多电子透明的颗粒以及类似藻青素或淀粉粒的细胞器以及羧酶体。色素组成方面，细胞最主要的色素是叶绿素a，β-胡萝卜素，玉米黄素，以及一种未知色素，分别占总色素比例为23.8%，26.1%，14.7% and 15.7%。经过16S rRNA以及nifH基因序列分析，此株系被鉴定为蓝杆藻。 |
| 英文摘要: |
| One strain of unicellular greenish algae embedded by mucilage was successfully isolated from equatorial area in the Indian Ocean. Microscopic observation, ultrastructure features and genetic identification confirmed that the strain was closely related to Cyanothece sp., which was a cyanobacteria species with great ecological significance. Cells were solitary with oval or bacilliform shapes. Diameters of this strain were relatively small, ranging from 2.5 to 6.5 μm on average. Ultrastructure of cells was simple. Thylakoids were arranged parietal and keritomized content were observed in the thylakoid region. Various electron-transparent granules with low electron-dense region as well as cyanophycin or glycogen granules-like organelle and carbonxysomes were also observed. For pigment composition, the dominant pigments were chlorophyll a, β-Carotene, Zeaxanthin and an unknown pigment, contributing 23.8%, 26.1%, 14.7% and 15.7% to total pigments respectively. The phylogenetic analysis of 16S rRNA gene and nifH gene confirmed that Strain EIO409 was closely related with Cyanothece sp.. |
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