施肥对盐沼沉积物微生物生物量和细菌群落组成的影响

马悦欣; 陶韦; 刘长发; 刘姣; 杨志平; 李晋; 刘继晨

快速检索：				高级检索

MA Yuexin,TAO Wei,LIU Changfa,LIU Jiao,YANG Zhiping,LI Jin,LIU Jichen.Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China[J].Acta Oceanologica Sinica,2017,36(6):80-88

施肥对盐沼沉积物微生物生物量和细菌群落组成的影响

Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China

投稿时间：2016-01-16 修订日期：2016-08-02

DOI：10.1007/s13131-017-1048-5

中文关键词: 施肥微生物生物量 16S rRNA基因丰度细菌群落盐沼

英文关键词:fertilization microbial biomass 16S rRNA gene abundance bacterial community salt marsh

基金项目:The National Natural Science Foundation of China under contract No. 41171389; the Public Science and Technology Research Funds Projects of Ocean under contract No. 201305043; Program for Liaoning Excellent Talents in University under contract No. LR2013035.

作者	单位	E-mail
马悦欣	农业部海洋水产增养殖学重点开放实验室, 大连 116023	mayuexin@dlou.edu.cn
陶韦	辽宁省高校近岸海洋环境科学与技术重点实验室, 大连 116023
刘长发	辽宁省高校近岸海洋环境科学与技术重点实验室, 大连 116023
刘姣	农业部海洋水产增养殖学重点开放实验室, 大连 116023
杨志平	大连汇新钛设备开发有限公司, 大连 116039
李晋	盘锦海洋与水产研究所, 盘锦 124010
刘继晨	农业部海洋水产增养殖学重点开放实验室, 大连 116023

摘要点击次数: 1329

全文下载次数: 958

中文摘要:

通过氯仿熏蒸提取法、实时荧光定量PCR和变性梯度凝胶电泳（DGGE）研究了施氮（N）对双台河口翅碱蓬生长区盐沼沉积物微生物生物量、细菌丰度和群落组成的影响。于2013年7月、8月、9月和11月植物生长季节采集和分析经不同N量（尿素：0.1、0.2、0.4和0.8 g/kg（以干沉积物中N计））和不同N形式（尿素、（NH₄）₂SO₄和NH₄NO₃各0.2 g/kg）1、2、3和4次处理后的沉积物及未经处理的对照样品。结果表明，N量和N形式对微生物生物量碳、微生物生物量氮和细菌丰度产生正影响。DGGE图谱显示，N量和N形式也影响细菌群落组成。研究表明，短期施氮能增加微生物生物量和细菌丰度，并且改变细菌群落结构。

英文摘要:

The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer (urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg (nitrogen content in sediment) and different forms of N fertilizers (urea, (NH₄)₂SO₄, and NH₄NO₃, each supplied at 0.2 g/kg (calculated by nitrogen). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community.

查看全文查看/发表评论下载PDF阅读器

关闭