不同氮源下南极冰藻Chlamydomonas sp.ICE-L的生物量、氮吸收与脂肪酸组成研究

安美玲; 王以斌; 刘芳明; 綦晓青; 郑洲; 叶乃好; 孙承君; 缪锦来

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AN Meiling,WANG Yibin,LIU Fangming,QI Xiaoqing,ZHENG Zhou,YE Naihao,SUN Chengjun,MIAO Jinlai.Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources[J].Acta Oceanologica Sinica,2017,36(2):105-110

不同氮源下南极冰藻Chlamydomonas sp.ICE-L的生物量、氮吸收与脂肪酸组成研究

Biomass, nutrient uptake and fatty acid composition of Chlamydomonas sp. ICE-L in response to different nitrogen sources

投稿时间：2015-12-12 修订日期：2016-04-14

DOI：10.1007/s13131-017-0984-4

中文关键词: 南极冰藻 Chlamydomonas sp.ICE-L 缺氮氮吸收多不饱和脂肪酸(PUFAs)

英文关键词:Antarctic ice alga Chlamydomonas sp. ICE-L nitrogen-deficiency condition nutrient uptake polyunsaturated fatty acids (PUFAs)

基金项目:The National Natural Science Foundation of China under contract No. 41576187; the National Natural Science Foundation of China-Shandong Joint Fund under contract No. U1406402; the Basic Scientific Fund for National Public Research Institutes of China under contract No. 2015G10; the Polar Strategic Foundation of China under contract No. 20150303; the Public Science and Technology Research Funds Projects of Ocean under contract No. 201405015; the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology and the Science under contract No. 2015ASKJ02; the Science and Technology Planning Project of Shandong Province under contract No. 2014GHY115003; the Major Projects of Independent Innovation Achievements Transformation in Shandong Province under contract No. 2014ZZCX06202; Qingdao Entrepreneurship and Innovation Pioneers Program under contract No. 15-10-3-15-(44)-zch.

作者	单位	E-mail
安美玲	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国青岛大学, 山东青岛, 226071, 中国
王以斌	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国青岛海洋科学与技术国家实验室, 山东青岛, 266235, 中国
刘芳明	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国青岛海洋科学与技术国家实验室, 山东青岛, 266235, 中国
綦晓青	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国
郑洲	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国青岛海洋科学与技术国家实验室, 山东青岛, 266235, 中国
叶乃好	中国水产科学研究院黄海水产研究所, 山东青岛, 226071, 中国
孙承君	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国
缪锦来	国家海洋局第一海洋研究所, 山东青岛, 266061, 中国青岛大学, 山东青岛, 226071, 中国青岛海洋科学与技术国家实验室, 山东青岛, 266235, 中国	miaojinlai@fio.org.cn

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中文摘要:

微藻培养过程中氮缺失有利于油脂和生物量的积累，然而不同氮源条件下微藻生长与生物量的研究有限，限制了生物油脂的相关研究。本文研究通过研究南极冰藻Chlamydomonas sp.ICE-L在不同氮源条件下的细胞生长与油脂积累，进一步探究其作为富集油脂微藻的潜力。研究发现：在含有NH₄CL的培养基中，Chlamydomonas sp.ICE-L生长速率最大；在含有NH₄NO₃的条件下，获得了最大干重量0.28 g/L。最高油脂含量0.21 g/g是在缺氮条件下获得，同时得到干重0.24 g/L。在多不饱和脂肪酸的产出方面，NH₄NO₃和NH₄Cl为氮源培养基时要好于缺氮和KNO₃培养基，在NH₄NO₃和NH₄Cl为氮源的培养条件下ICE-L胞内C18：3和C20：5的含量高。比较而言，缺氮和KNO₃培养基时C16：0、C18：1和C18：2的含量要高。

英文摘要:

Nitrogen removal from media by microalgae provides the potential benefit of producing lipids for biodiesel and biomass. However, research is limited on algal growth and biomass under different nitrogen sources and provides little insight in terms of biofuel production. We studied the influences of nitrogen sources on cell growth and lipid accumulation of Chlamydomonas sp. ICE-L, one of a promising oil rich micro algal species. Chlamydomonas sp. ICE-L grown in NH₄Cl medium had maximum growth rate. While the highest dry biomass of 0.28 g/L was obtained in media containing NH₄NO₃, the highest lipid content of 0.21 g/g was achieved under nitrogen-deficiency condition with a dry biomass of 0.24 g/L. In terms of total polyunsaturated fatty acids (PUFAs) production, NH₄NO₃ and NH₄Cl media performed better than nitrogen-deficiency and KNO₃ media. Furthermore, NH₄NO₃ and NH₄Cl media elucidated better results on C18:3 and C20:5 productions while KNO₃ and -N conditions were better in C16:0, C18:1 and C18:2, comparatively.

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