| HE Yuying,DU Ying,LI Jian,LIU Ping,WANG Qingyin,LI Zhaoxia.Analysis of DNA methylation in different tissues of Fenneropenaeus chinensis from the wild population and Huanghai No. 1[J].Acta Oceanologica Sinica,2015,34(12):175-180 |
| 中国对虾选育群体“黄海1号”和野生群体不同组织基因组DNA甲基化的MSAP分析 |
| Analysis of DNA methylation in different tissues of Fenneropenaeus chinensis from the wild population and Huanghai No. 1 |
| 投稿时间：2014-08-01 修订日期：2014-12-15 |
| DOI：10.1007/s13131-015-0765-x |
| 中文关键词: MSAP DNA甲基化 中国对虾 |
| 英文关键词:methylation sensitive amplification polymorphism DNA methylation Fenneropenaeus chinensis |
| 基金项目:The National Natural Science Foundation of China under contract No. 31172401; the Independent Innovation Industry in Shandong Province Special of China under contract No. 2013CXC80202; China Agriculture Research System under contract No. CARS-47. |
| 作者 | 单位 | E-mail | | 何玉英 | 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所, 青岛 266071
中国海洋大学海洋生命学院, 青岛 266003 | | | 杜盈 | 青岛农业大学海洋科学与工程学院, 青岛 266109 | | | 李健 | 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所, 青岛 266071
中国海洋大学海洋生命学院, 青岛 266003 | lijian@ysfri.ac.cn | | 刘萍 | 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所, 青岛 266071 | | | 王清印 | 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所, 青岛 266071 | | | 李朝霞 | College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China | |
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| 中文摘要: |
| DNA甲基化在调节动物生长发育和组织分化中发挥了重要作用。本研究主要从酶切、预扩和选扩等方面优化了中国对虾DNA甲基化分析的MSAP技术,给出了适合中国对虾MSAP分析的最近反应程序和体系,并采用该技术分别对中国对虾选育群体"黄海1号"和野生群体的肌肉、鳃和血细胞三种组织基因组DNA的CCGG甲基化水平进行分析。研究结果表明,中国对虾野生群体肌肉、鳃和血细胞的DNA甲基化比例分别为23.1%、22.3%和19.7%,选育群体"黄海1号"肌肉、鳃和血液的甲基化比例分别为21.4%、19.6%和18.9%。鳃组织的DNA甲基化水平在两群体中差异极显著(P <0.01),肌肉间的甲基化水平差异显著(P <0.05),而血细胞中甲基化水平差异不显著(P >0.05)。中国对虾野生群体和"黄海1号"同一组织间的甲基化水平不同(P ≤ 0.05),而不同组织间的甲基化水平也各不相同(P ≤ 0.05)。DNA甲基化多态性分析表明,野生群体和选育群体"黄海1号"的鳃和血细胞组织的甲基化多态性比例变化明显,而肌肉组织甲基化水平较稳定,这些变化趋势与CCGG位点的甲基化和去甲基化有关。 |
| 英文摘要: |
| DNA methylation plays an important role in the regulation of gene expression during biological development and tissue differentiation in eukaryotes. A methylation sensitive amplification polymorphism (MSAP) including digestion, pre-selective amplification and selective amplification was optimized to compare the levels of DNA cytosine methylation at CCGG sites in muscle, gill and hemocyte from the wild populations and the selective breeding of Huanghai No. 1 of Fenneropenaeus chinensis, respectively. Significant differences in cytosine methylation levels among three tissues in two populations were detected. The average DNA methylation ratios in muscle, gill and hemocyte of the wild population were 23.1%, 22.3% and 19.7%, while those were 21.4%, 19.6%, and 18.9% in Huanghai No. 1, respectively. The DNA methylation levels of gill from the two populations were highly significant (P <0.01), the difference of muscle was significant (P <0.05), while in hemocyte, there were no significant differences (P >0.05). DNA polymorphic methylation of gill and hemocyte between the wild population and Huanghai No. 1 varies to some extent, while those of muscle kept in a balanced degree. Furthermore, polymorphic methylation was associated with demethylation and methylation of CCGG loci. |
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