| HE Hui,ZHEN Yu,MI Tiezhu,LU Xinglan,YU Zhigang.Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea[J].Acta Oceanologica Sinica,2015,34(8):83-92 |
| 东海表层沉积物中氨氧化微生物群落的空间分布和季节性变化 |
| Seasonal and spatial distribution of ammonia-oxidizing microorganism communities in surface sediments from the East China Sea |
| 投稿时间：2014-05-13 修订日期：2014-10-22 |
| DOI：10.1007/s13131-015-0710-z |
| 中文关键词: 氨氧化微生物 沉积物 东海 |
| 英文关键词:ammonia-oxidizing microorganisms sediment East China Sea |
| 基金项目:The National Natural Science Foundation of China under contract No. 40920164004; the National Basic Research Program (973 Program) of China under contract No. 2011CB403602; the Foundation for Innovative Research Groups of the National Natural Science Foundation of China under contract No. 41221004. |
| 作者 | 单位 | E-mail | | 贺惠 | 中国海洋大学海洋生命学院, 青岛, 266003 | | | 甄毓 | 中国海洋大学海洋环境与生态教育部重点实验室, 青岛, 266100
中国海洋大学环境科学与工程学院, 青岛, 266100 | zhenyu@ouc.edu.cn | | 米铁柱 | 中国海洋大学海洋环境与生态教育部重点实验室, 青岛, 266100
中国海洋大学环境科学与工程学院, 青岛, 266100 | | | 路兴岚 | 中国海洋大学环境科学与工程学院, 青岛, 266100 | | | 于志刚 | 中国海洋大学海洋化学理论与工程技术教育部重点实验室, 青岛, 266100 | |
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| 中文摘要: |
| 氨氧化在海洋沉积物的氮循环过程中发挥着重要作用.本实验以氨单加氧酶α亚基(amoA)为目的基因,采用实时定量PCR并结合环境因子分析的方法,研究了东海表层沉积物中氨氧化古菌(AOA)和β-氨氧化细菌(β-AOB)群落的空间分布和季节性变化.定量PCR结果显示,所有采样站位中,β-AOB amoA基因拷贝数均高于AOA amoA基因拷贝数.它们与环境因子均存在相关性.4月AOA amoA基因拷贝数与温度和亚硝酸盐浓度明显正相关(p< 0.05);8月β-AOB amoA基因拷贝数与盐度极显著负相关(p< 0.01),而与铵盐浓度明显正相关(p< 0.05).随着盐度升高,β-AOB和AOA的比值有降低趋势,表明β-AOB在铵盐浓度高的区域占优势,而AOA更适合生活在高盐度的海域中 |
| 英文摘要: |
| Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea (AOA) and betaproteobacteria (β-AOB) in surface sediments from the East China Sea (ECS) were investigated using ammonia monooxygenase α subunit (amoA) gene. In order to characterize the community of AOA and β-AOB, real-time quantitative polymerase chain reaction (qPCR) was carried out in this study, along with environmental parameters. The abundance of β-AOB amoA gene (2.17×106-4.54×107 copy numbers per gram wet weight sediment) was always greater than that of AOA amoA gene (2.18×105-9.89×106 copy numbers per gram wet weight sediment) in all sampling stations. The qPCR results were correlated with environmental parameters. AOA amoA gene copy numbers in April were positively related to temperature and nitrite concentration (p <0.05). β-AOB amoA gene copy numbers in August correlated negatively with salinity (p <0.01), and correlated positively with ammonium concentration (p <0.05). With the increase of salinity, the amoA gene copy ratio of AOB to AOA had a tendency to decrease, which suggested β-AOB dominated in the area of high level ammonium and AOA preferred high salinity area. |
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