| Wu Yuehong,Cao Yi,Wang Chunsheng,Wu Min,Aharon Oren,Xu Xuewei.Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge[J].Acta Oceanologica Sinica,2014,33(10):94-104 |
| 西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性 |
| Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge |
| 投稿时间：2013-03-25 修订日期：2013-11-02 |
| DOI：10.1007/s13131-014-0544-0 |
| 中文关键词: 深海 热液喷口 微生物多样性 16S rRNA nifH |
| 英文关键词:deep-sea hydrothermal vent microbial diversity 16S rRNA gene nifH gene |
| 基金项目:The China Ocean Mineral Resources R & D Association (COMRA) Special Foundation under contract No. DY125-15-R-03; the National Natural Science Foundation of China under contract Nos 41276173 and 41206104; the Scientific Research Fund of the Second Institute of Oceanography, State Oceanic Administration under contract No. JT1011. |
| 作者 | 单位 | E-mail | | 吴月红 | 国家海洋局第二海洋研究所海洋生态与生物地球化学实验室, 杭州 310012, 中国 | | | 曹佚 | 中科院微生物所微生物资源国家重点实验室, 北京310058, 中国 | | | 王春生 | 国家海洋局第二海洋研究所海洋生态与生物地球化学实验室, 杭州 310012, 中国 | | | 吴敏 | 浙江大学生命科学学院院, 杭州310012, 中国 | | | Aharon Oren | 耶路撒冷希伯来大学生命科学学院亚历山大贝尔西曼研究所植物和环境科学部门, 耶路撒冷91904, 以色列
耶路撒冷希伯来大学爱德蒙·
J·
莎拉学院, 耶路撒冷吉瓦特·
拉姆91904, 以色列 | | | 许学伟 | 国家海洋局第二海洋研究所海洋生态与生物地球化学实验室, 杭州 310012, 中国 | xuxw@sio.org.cn |
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| 中文摘要: |
| 沉积物样品来自西南印度洋深海热液硫化物区，水深为2951米。通过扩增其16S rRNA 和nifH基因研究微生物群落结构。古菌克隆主要为MBGE（Marine Benthic Group E）和MGI (Marine GroupⅠ)类群，分别属于广古菌门和奇古菌门。超过一半的细菌克隆属于变形杆菌（Proteobacteria），且大部分为γ-变形菌纲。未检测到ε-变形杆菌纲的克隆。此外还检测到放线菌门、拟杆菌门、浮霉菌门、酸杆菌门、消化螺旋菌门、绿弯菌门、绿菌门、衣原体门、疣微菌门和几个候补类群（OD1、 OP11、WS3、TM6），表明这些类群存在于热液硫化物环境。固氮基因（nifH）研究揭示了热液硫化物环境存在生物氮固定过程。系统发育分析表明该环境只存在类群I和类群III固氮基因。这和16S rRNA系统发育分析结果一致，表明细菌在热液硫化物环境氮固定过程中可能发挥了主要作用。 |
| 英文摘要: |
| A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilon Proteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III nifH were present. This is consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment. |
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