| CHU Wuying,YU Lian,QIAN Ronghua,MENG Tao,ZHOU Ruixue,FU Guihong,CHEN Jia,ZHANG Jianshe.Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea[J].Acta Oceanologica Sinica,2008,(5):126-133 |
| Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea |
| Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea |
| 投稿时间：2007-01-23 修订日期：2007-11-12 |
| DOI： |
| 中文关键词: globin intergenic intragenic intron large yellow croaker promoter regulation |
| 英文关键词:globin intergenic intragenic intron large yellow croaker promoter regulation |
| 基金项目:The National Natural Science Foundation of China under contract Nos 30640015 and 30771644, and the Major Program of Zhejiang Province of China under contract No. 2005C23085. |
| 作者 | 单位 | E-mail | | CHU Wuying | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | YU Lian | Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | QIAN Ronghua | Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China | | | MENG Tao | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | ZHOU Ruixue | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | FU Guihong | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | CHEN Jia | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | | | ZHANG Jianshe | Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China | jzhang@ccsu.cn |
|
| 摘要点击次数: 934 |
| 全文下载次数: 629 |
| 中文摘要: |
| One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the αand β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked αand β-globin genes. |
| 英文摘要: |
| One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the αand β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked αand β-globin genes. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|