| LU Yongzhong,ZHANG Xuecheng.Analysis on promoter elements of cpc operon from Arthrospira platensis[J].Acta Oceanologica Sinica,2008,(1):85-91 |
| Analysis on promoter elements of cpc operon from Arthrospira platensis |
| Analysis on promoter elements of cpc operon from Arthrospira platensis |
| 投稿时间：2006-12-20 修订日期：2007-10-11 |
| DOI： |
| 中文关键词: c-phycocyanin Smart Race transcription promoter cyanobacterium |
| 英文关键词:c-phycocyanin Smart Race transcription promoter cyanobacterium |
| 基金项目:The National Natural Science Foundation of China under contract No.30471317. |
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| 中文摘要: |
| On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E.coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the -285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ. |
| 英文摘要: |
| On the basis of bioinformatics analysis, six putative promoters were isolated from the upstream sequence of c-phycocyanin gene of Arthrospira platensis FACHB341 through site-directed mutagenesis, and the transcriptional pattern of c-phycocyanin gene was determined by SmartRace. Results showed that each isolated promoter drived the expression of gfp in E.coli, and Promoter Ⅲ was the strongest one according to the GFP level. Only one transcript of c-phycocyanin gene was found under experimental conditions, and the transcription start site G was located at the -285 bp upstream of the start codon, from which it could be inferred that the transcript was from the promotion of Promoter Ⅰ. |
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