| HE Shanying,YU Zhigang,MI Tiezhu.Development of a real-time PCR method for Thalassiosira rotula rapid detection[J].Acta Oceanologica Sinica,2007,(2):133-139 |
| Development of a real-time PCR method for Thalassiosira rotula rapid detection |
| Development of a real-time PCR method for Thalassiosira rotula rapid detection |
| 投稿时间：2006-03-07 修订日期：2006-11-01 |
| DOI： |
| 中文关键词: red tide Thalassiosira rotula fluorescent quantitative PCR 18S rDNA |
| 英文关键词:red tide Thalassiosira rotula fluorescent quantitative PCR 18S rDNA |
| 基金项目: |
|
| 摘要点击次数: 906 |
| 全文下载次数: 605 |
| 中文摘要: |
| Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula.A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula.The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. |
| 英文摘要: |
| Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula.A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula.The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |