| WANG Dianliang,LIU Wanshun,HAN Baoqin.Enzymatic properties of UFE, a novel marine fibrinolytic enzyme[J].Acta Oceanologica Sinica,2007,(2):84-93 |
| Enzymatic properties of UFE, a novel marine fibrinolytic enzyme |
| Enzymatic properties of UFE, a novel marine fibrinolytic enzyme |
| 投稿时间：2006-04-03 修订日期：2006-08-28 |
| DOI： |
| 中文关键词: marine animal fibrinolytic enzyme protease enzyme properties |
| 英文关键词:marine animal fibrinolytic enzyme protease enzyme properties |
| 基金项目: |
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| 中文摘要: |
| A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered.The enzymatic properties of UFE were further investigated.As a low molecular mass protein, UFE appeared to be very stable to heat and pH.When temperature was below 50℃, the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60℃, the remnant enzyme activity began to decrease rapidly.UFE was quite stable in the range of pH value from 3 to 12, especially in slightly alkaline pH value.Mn2+, Cu2+ and Fe2+ ions were activators of UFE, while Fe3+ and Ag+ ions were inhibitors of UFE.Fe2+ ion along with Fe3+ ion might regulate UFE activity in vivo.The optimum pH and temperature of UFE were about 8 and 50℃, respectively.Other characteristics of this enzyme were also studied.Systematic research results are significant when UFE is applied for medical and industrial purposes. |
| 英文摘要: |
| A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered.The enzymatic properties of UFE were further investigated.As a low molecular mass protein, UFE appeared to be very stable to heat and pH.When temperature was below 50℃, the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60℃, the remnant enzyme activity began to decrease rapidly.UFE was quite stable in the range of pH value from 3 to 12, especially in slightly alkaline pH value.Mn2+, Cu2+ and Fe2+ ions were activators of UFE, while Fe3+ and Ag+ ions were inhibitors of UFE.Fe2+ ion along with Fe3+ ion might regulate UFE activity in vivo.The optimum pH and temperature of UFE were about 8 and 50℃, respectively.Other characteristics of this enzyme were also studied.Systematic research results are significant when UFE is applied for medical and industrial purposes. |
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