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FANG Hai,ZHANG Xiaojun,CHEN Cuizhen,JIN Xiaomin,WANG Xiuyun.Studies on the edwardsiellosis and characterization of pathogenic bacteria from diseased flounder (Paralichthys olivaceus L.) and turbot (Scophthalmus maximus L.)[J].Acta Oceanologica Sinica,2006,(3):138-147
Studies on the edwardsiellosis and characterization of pathogenic bacteria from diseased flounder (Paralichthys olivaceus L.) and turbot (Scophthalmus maximus L.)
Studies on the edwardsiellosis and characterization of pathogenic bacteria from diseased flounder (Paralichthys olivaceus L.) and turbot (Scophthalmus maximus L.)
投稿时间:2005-12-29  修订日期:2006-03-30
DOI:
中文关键词:  flounder (Paralichthys olivaceus L.)  turbot (Scophthalmus maximus L.)  edwardsiellosis  pathogenic bacteria
英文关键词:flounder (Paralichthys olivaceus L.)  turbot (Scophthalmus maximus L.)  edwardsiellosis  pathogenic bacteria
基金项目:
作者单位E-mail
FANG Hai Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China fanghaihb@163.com 
ZHANG Xiaojun Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China  
CHEN Cuizhen Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China  
JIN Xiaomin Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China  
WANG Xiuyun Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China  
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中文摘要:
      Edwardsiellosis of flounder and turbot occurring in different mariculture farms during 2001~2004 was examined, including the conditions of disease occurrence, clinical signs and pathological changes. The results showed that all diseased fishes expressed bacterial septicaemia. A total of 148 strains were identified using a combination of traditional physiological and biochemical tests and partial 16S rRNA gene analysis. In addition, the mole fraction G + C ratio of the DNA of representative strain of isolates and serum homology were detected, and pathogenicity tests of isolates were conducted by experimental infection. The results revealed that 148 strains were identified as E. tarda of genus Edwardsiella, all the isolates are of serologic similarity, and have strong pathogenicity to flounder and turbot.
英文摘要:
      Edwardsiellosis of flounder and turbot occurring in different mariculture farms during 2001~2004 was examined, including the conditions of disease occurrence, clinical signs and pathological changes. The results showed that all diseased fishes expressed bacterial septicaemia. A total of 148 strains were identified using a combination of traditional physiological and biochemical tests and partial 16S rRNA gene analysis. In addition, the mole fraction G + C ratio of the DNA of representative strain of isolates and serum homology were detected, and pathogenicity tests of isolates were conducted by experimental infection. The results revealed that 148 strains were identified as E. tarda of genus Edwardsiella, all the isolates are of serologic similarity, and have strong pathogenicity to flounder and turbot.
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