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HUANG Xiaoting,BAO Zhenmin,BI Ke,HU Jingjie,ZHANG Can,ZHANG Quanqi,HU Xiaoli.Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri[J].Acta Oceanologica Sinica,2006,(3):108-115
Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri
Chromosomal localization of the major ribosomal RNA genes in scallop Chlamys farreri
投稿时间:2006-01-09  修订日期:2006-04-18
DOI:
中文关键词:  rDNA  Ag-NORs  FISH  Chlamys farreri
英文关键词:rDNA  Ag-NORs  FISH  Chlamys farreri
基金项目:
作者单位E-mail
HUANG Xiaoting Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
BAO Zhenmin Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
BI Ke Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
HU Jingjie Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
ZHANG Can Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
ZHANG Quanqi Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China  
HU Xiaoli Laboratory of Marine Genetics and Breedings, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China hxl707@ouc.edu.cn 
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中文摘要:
      The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization (FISH) with 18S-28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentric chromosome 10.After silverstaining, nucleolus organizer regions (NORs) could be observed on the telomere of the short arm of chromosome 10.However,one metaphase spread displayed an additional silver spot on the short arm of subtelocentric chromosome 12.
英文摘要:
      The chromosomes of Chlamys farreri were analyzed by means of silver staining and fluorescence in situ hybridization (FISH) with 18S-28S rDNA probe. Probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, ITS2 between 5.8S and 28S ribosomal RNA gene and 5.8S rRNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals were located on the short arm of subtelocentric chromosome 10.After silverstaining, nucleolus organizer regions (NORs) could be observed on the telomere of the short arm of chromosome 10.However,one metaphase spread displayed an additional silver spot on the short arm of subtelocentric chromosome 12.
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