| LIU Yongjian,YANG Guanpin,GUAN Xiaojing,MEN Rongxin.Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing[J].Acta Oceanologica Sinica,2006,(2):125-134 |
| Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing |
| Genetic diversity and its seasonal variation of Jiaozhou Bay phytoplankton determined by rbcL gene sequencing |
| 投稿时间：2005-10-23 修订日期：2006-01-27 |
| DOI： |
| 中文关键词: phytoplankton ribulose-1 5-bisphosphate carboxynase/oxygenase large subunit gene(rbcL) community structure seasonal variation |
| 英文关键词:phytoplankton ribulose-1 5-bisphosphate carboxynase/oxygenase large subunit gene(rbcL) community structure seasonal variation |
| 基金项目: |
| 作者 | 单位 | E-mail | | LIU Yongjian | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | | | YANG Guanpin | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | yguanpin@mail.ouc.edu.cn | | GUAN Xiaojing | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | | | MEN Rongxin | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | |
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| 中文摘要: |
| Ribulose-1, 5-bisphosphate carboxynase/oxygenase large subunit gene (rbcL) of Jiaozhou Bay phytoplankton was amplified from spring, summer and autumn surface seawater DNAs and cloned respectively.About 50 clones were randomly selected from each library and sequenced.If identical amino acid sequences are considered as the same operational taxonomy unit (OTU), 61 OTUs are identified according to inferred amino acid sequences, among them, 21 from spring seawater, 15 from summer seawater and 25 from autumn seawater.Shannon index calculated from OTU abundances reflects the genetic diversity of a community.The indexes of spring, summer and autumn surface seawater phytoplankton are 2.69, 2.44 and 2.76 respectively, indicating that phytoplankton genetic diversity of autumn seawater is the richest.Seasonal variation of phytoplankton community is significant; the community compositions of three seasons are almost completely different except for the two OTUs shared by summer and autumn.Surface seawater phytoplankton communities are possibly metacommunities different spatially and temporally. |
| 英文摘要: |
| Ribulose-1, 5-bisphosphate carboxynase/oxygenase large subunit gene (rbcL) of Jiaozhou Bay phytoplankton was amplified from spring, summer and autumn surface seawater DNAs and cloned respectively.About 50 clones were randomly selected from each library and sequenced.If identical amino acid sequences are considered as the same operational taxonomy unit (OTU), 61 OTUs are identified according to inferred amino acid sequences, among them, 21 from spring seawater, 15 from summer seawater and 25 from autumn seawater.Shannon index calculated from OTU abundances reflects the genetic diversity of a community.The indexes of spring, summer and autumn surface seawater phytoplankton are 2.69, 2.44 and 2.76 respectively, indicating that phytoplankton genetic diversity of autumn seawater is the richest.Seasonal variation of phytoplankton community is significant; the community compositions of three seasons are almost completely different except for the two OTUs shared by summer and autumn.Surface seawater phytoplankton communities are possibly metacommunities different spatially and temporally. |
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