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LI Huirong,YU Yong,CHEN Bo,ZENG Yinxin,REN Daming.Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin[J].Acta Oceanologica Sinica,2005,(6):153-161
Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin
Molecular genetic diversity of bacteria in the bottom section of arctic sea ice from the Canada Basin
投稿时间:2005-07-26  修订日期:2005-10-23
DOI:
中文关键词:  sea ice  Arctic Ocean  bacteria  16S rDNA  phylogenetic analysis
英文关键词:sea ice  Arctic Ocean  bacteria  16S rDNA  phylogenetic analysis
基金项目:
作者单位E-mail
LI Huirong State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China lihuirong72@163.com 
YU Yong State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China  
CHEN Bo State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China  
ZENG Yinxin State Oceanic Administration Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China  
REN Daming State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China  
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中文摘要:
      PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α-proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples.
英文摘要:
      PCR-DGGE approach was used to analyze bacterial diversity in the bottom section of seven arctic sea ice samples colleted from the Canada Basin. Thirty-two 16S rDNA sequences were obtained from prominent DGGE bands. The closest relatives of these sequences are found to be those of cultivated or uncultured bacteria from antarctic or arctic sea ice. Phylogenetic analysis clustered these sequences or phylotypes within α-proteobacteria, γ-proteobacteria and CFB (cytophaga-flexibacter-bacteroides) group. Sequences belonging to γ-proteobacteria were dominant and members of the CFB group were highly abundant. It was suggested that the CFB group was the representative of the bottom section of sea ice samples.
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