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MU Chunhua,BAO Zhenmin,CHEN Gang,HU Jingjie,HAO Lujiang,QI Zizhong,LI Guangxue.Bacterial diversity in the sediments collected from the Shikoku Basin[J].Acta Oceanologica Sinica,2005,(3):114-121
Bacterial diversity in the sediments collected from the Shikoku Basin
Bacterial diversity in the sediments collected from the Shikoku Basin
投稿时间:2004-12-11  修订日期:2005-05-20
DOI:
中文关键词:  bacteria diversity  deep-sea sediment  Shikoku Basin  Northwest Pacific Ocean
英文关键词:bacteria diversity  deep-sea sediment  Shikoku Basin  Northwest Pacific Ocean
基金项目:
作者单位E-mail
MU Chunhua Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China  
BAO Zhenmin Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China zmba@ouc.edu.cn 
CHEN Gang Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China  
HU Jingjie Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China  
HAO Lujiang Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China  
QI Zizhong Laboratory of Marine Genetics and Breedings(MGB), Division of Life Science and Technology, Ocean University of China, Qing-dao 266003, China  
LI Guangxue College of Marine Geosciences, Ocean University of China, Qingdao 266003, China  
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中文摘要:
      Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups:Proteobacteria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.
英文摘要:
      Diversity of bacteria was studied in deep-sea sediments from the Shikoku Basin in the Northwest Pacific Ocean by PCR, RFLP and sequence analysis of 16S rDNA and comparing with Genbank database. Based on the RFLP profile generated, 77 clones from the 16S rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups:Proteobacteria (62.96%), Chloroflexi (14.81%), Planctomycetes (14.81%) and Acidobacteria (7.41%). Among all sequenced clones, 6 were related to the sulfur or sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.
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