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WANG Jun,ZHANG Wen,SU Yongquan,DING Shaoxiong.Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences[J].Acta Oceanologica Sinica,2004,(4):709-716
Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences
Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences
投稿时间:2003-10-11  修订日期:2004-01-20
DOI:
中文关键词:  S rRNA  Neobenedenia  sequence analysis
英文关键词:S rRNA  Neobenedenia  sequence analysis
基金项目:
作者单位E-mail
WANG Jun College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China junw@xmu.edu.cn 
ZHANG Wen College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China  
SU Yongquan College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China  
DING Shaoxiong College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China  
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中文摘要:
      The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varying in 337bp accounting for 0.3% genetic difference,from the relative species N.girellae and N.melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni sampled in different areas,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.,B.lutjani,B.rohdei and B.seriolae) range from 2.08% to11.73%.In addition,UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity,whether they belong to a single species or not are still undefined,and the more genes of them should be further investigated,in combination with the systematical and detailed morphological study.
英文摘要:
      The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda,Neobenedenia girellae and N.melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced.The results show that the comparison of 28S rRNA sequences,with only a base varying in 337bp accounting for 0.3% genetic difference,from the relative species N.girellae and N.melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%,higher than that of 99.41% for the single species N.melleni sampled in different areas,and the intraspecific divergence of N.melleni is 0.59%.Meanwhile,the interspecific differences between the two Neobenedenia and three Benedenia (i.e.,B.lutjani,B.rohdei and B.seriolae) range from 2.08% to11.73%.In addition,UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other.Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity,whether they belong to a single species or not are still undefined,and the more genes of them should be further investigated,in combination with the systematical and detailed morphological study.
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