| ZENG Yonghui,JIAO Nianzhi,CAI Haiyuan,CHEN Xihan,WEI Chaoling.Phylogenetic diversity of ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea[J].Acta Oceanologica Sinica,2004,(4):673-685 |
| Phylogenetic diversity of ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea |
| Phylogenetic diversity of ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea |
| 投稿时间：2004-01-02 修订日期：2004-09-22 |
| DOI： |
| 中文关键词: RubisCO rbcL gene phylogenetic analysis clone library DGGE East China Sea |
| 英文关键词:RubisCO rbcL gene phylogenetic analysis clone library DGGE East China Sea |
| 基金项目: |
| 作者 | 单位 | E-mail | | ZENG Yonghui | Key Laboratory for Marine Environmental Science of the Ministry of Education, Environmental Science ResearchCenter, Xiamen University, Xiamen 361005, China | | | JIAO Nianzhi | Key Laboratory for Marine Environmental Science of the Ministry of Education, Environmental Science ResearchCenter, Xiamen University, Xiamen 361005, China | jiao@xmu.edu.cn | | CAI Haiyuan | Key Laboratory for Marine Environmental Science of the Ministry of Education, Environmental Science ResearchCenter, Xiamen University, Xiamen 361005, China | | | CHEN Xihan | Key Laboratory for Marine Environmental Science of the Ministry of Education, Environmental Science ResearchCenter, Xiamen University, Xiamen 361005, China | | | WEI Chaoling | Key Laboratory for Marine Environmental Science of the Ministry of Education, Environmental Science ResearchCenter, Xiamen University, Xiamen 361005, China | |
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| 中文摘要: |
| Phylogenetic diversity of Form I and Form Ⅱ ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large subunit (rbcL) genes in the inshore and offshore areas of the East China Sea were investigated.Two new primer sets were designed for amplifying partial sequences of rbcL genes from Proteobacteria.Four rbcL gene clone libraries were constructed by amplification and cloning of approximately 640~800 bp sequences of bacterioplankton populations.The method of screening library by denaturing gradient gel electrophoresis (DGGE) was introduced.The results show that the diversity of Form I is higher in offshore waters with higher salinity and lower productivity,while that of Form Ⅱ is higher at the inshore station where salinity is lower and productivity is higher.Several clusters of sequences obtained are deeply rooted and show low similarity (60%~78%) to the known rbcL in existing databases.The degree of diversity of rbcL genes is directly related to environmental variables,including temperature,salinity,pH,dissolved oxygen,etc.These results indicate that rbcL gene can be used as an effective indicator for genetic diversity and population variability of bacterioplankton with the ability of carbon dioxide fixation in the sea. |
| 英文摘要: |
| Phylogenetic diversity of Form I and Form Ⅱ ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large subunit (rbcL) genes in the inshore and offshore areas of the East China Sea were investigated.Two new primer sets were designed for amplifying partial sequences of rbcL genes from Proteobacteria.Four rbcL gene clone libraries were constructed by amplification and cloning of approximately 640~800 bp sequences of bacterioplankton populations.The method of screening library by denaturing gradient gel electrophoresis (DGGE) was introduced.The results show that the diversity of Form I is higher in offshore waters with higher salinity and lower productivity,while that of Form Ⅱ is higher at the inshore station where salinity is lower and productivity is higher.Several clusters of sequences obtained are deeply rooted and show low similarity (60%~78%) to the known rbcL in existing databases.The degree of diversity of rbcL genes is directly related to environmental variables,including temperature,salinity,pH,dissolved oxygen,etc.These results indicate that rbcL gene can be used as an effective indicator for genetic diversity and population variability of bacterioplankton with the ability of carbon dioxide fixation in the sea. |
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