| Lin Xuezheng,Yang Xiuxia,Bian Ji,Huang Xiaohang.Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean[J].Acta Oceanologica Sinica,2003,(4):643-649 |
| Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
| Study on low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from deep sea of Southern Ocean |
| 投稿时间：2003-03-18 修订日期：2003-08-12 |
| DOI： |
| 中文关键词: Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
| 英文关键词:Southern Ocean deep sea psychrophilic bacteria low-temperature lipase |
| 基金项目:This study was supported by 10th five year plan(DY105040203) of China Ocean Mineral Resources R&D Association |
| 作者 | 单位 | | Lin Xuezheng | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | Yang Xiuxia | Fishery College, Ocean University of China, Qingdao 266003, China | | Bian Ji | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | Huang Xiaohang | First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China |
|
| 摘要点击次数: 889 |
| 全文下载次数: 714 |
| 中文摘要: |
| A strain of psychrophilic bacterium,2-5-10-1,which produces low-temperature lipase,is isolated from the deep sea of Prydz Bay in Southern Ocean.The highest lipase secretion of this strain is observed at 5℃ and this temperature is also for optimal growth.Tween 80 and olive oil enhance secretion of lipase.The optimal temperature and pH for lipase activity are 35℃ and 7.5℃ respectively.At 0℃,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,more than 50% activity lost after incubation at 60℃ for 15 min.EDTA has no effect on lipase activity,indicating the lipase activity is independent of divalent cation.In contrast,the lipase activity is inhibited drastically by Cu2+ and Zn2+. |
| 英文摘要: |
| A strain of psychrophilic bacterium,2-5-10-1,which produces low-temperature lipase,is isolated from the deep sea of Prydz Bay in Southern Ocean.The highest lipase secretion of this strain is observed at 5℃ and this temperature is also for optimal growth.Tween 80 and olive oil enhance secretion of lipase.The optimal temperature and pH for lipase activity are 35℃ and 7.5℃ respectively.At 0℃,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,more than 50% activity lost after incubation at 60℃ for 15 min.EDTA has no effect on lipase activity,indicating the lipase activity is independent of divalent cation.In contrast,the lipase activity is inhibited drastically by Cu2+ and Zn2+. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|