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Wang Bin,Yang Feng,Chen Rongzhong,Xu Xun.Detection of Hepatitis A virus in shellfish by reverse transcription PCR[J].Acta Oceanologica Sinica,1997,(4):527-535
Detection of Hepatitis A virus in shellfish by reverse transcription PCR
Detection of Hepatitis A virus in shellfish by reverse transcription PCR
投稿时间:1996-10-20  修订日期:1997-01-05
DOI:
中文关键词:  Shellfish HAV  RT-PCR  competitive
英文关键词:Shellfish HAV  RT-PCR  competitive
基金项目:
作者单位
Wang Bin Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 
Yang Feng Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 
Chen Rongzhong Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 
Xu Xun Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 
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中文摘要:
      A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR.
英文摘要:
      A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR.
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