| Wang Bin,Yang Feng,Chen Rongzhong,Xu Xun.Detection of Hepatitis A virus in shellfish by reverse transcription PCR[J].Acta Oceanologica Sinica,1997,(4):527-535 |
| Detection of Hepatitis A virus in shellfish by reverse transcription PCR |
| Detection of Hepatitis A virus in shellfish by reverse transcription PCR |
| 投稿时间:1996-10-20 修订日期:1997-01-05 |
| DOI: |
| 中文关键词: Shellfish HAV RT-PCR competitive |
| 英文关键词:Shellfish HAV RT-PCR competitive |
| 基金项目: |
| 作者 | 单位 | | Wang Bin | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Yang Feng | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Chen Rongzhong | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | Xu Xun | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| 中文摘要: |
| A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR. |
| 英文摘要: |
| A reverse transcription PCR (RT-PCR) method was used to detect the Hepatitis A virus in clam tissues The RNA template for PCR was prepared by a modified described previously (Yang and Xu, 1993).Its recovery wes determined by DNA hybridization.The sensitivity of RT-PCR and the quantitation of HAV were determined by quantitative competitive PCR. |
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