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刘晨临,王秀良,林学政.南极红藻Iridaea cordataCurdiea racovitzae转录组分析及其极端光环境适应相关基因的挖掘[J].海洋学报,2020,42(10):110-120
南极红藻Iridaea cordataCurdiea racovitzae转录组分析及其极端光环境适应相关基因的挖掘
De nova transcriptome analysis and mining extreme light environments acclimation responding genes of Antarctic seaweed Iridaea cordata (Gigartinales, Rhodophyta) and Curdiea racovitzae (Gracilariaceae, Rhodophyta)
投稿时间:2019-07-02  修订日期:2019-11-18
DOI:10.3969/j.issn.0253-4193.2020.10.011
中文关键词:  南极红藻  转录组  极端光环境适应  Iridaea cordata  Curdiea racovitzae
英文关键词:Antarctic red algae  transcriptome  extreme light environmental acclimation  Iridaea cordata  Curdiea racovitzae
基金项目:国家自然科学基金(41276203);国家南极观测监测网运维与管理项目(JDKC0518013)。
作者单位
刘晨临 自然资源部第一海洋研究所山东 青岛 266061
青岛海洋科学与技术试点国家实验室 海洋生物学与生物技术实验室山东 青岛 266237 
王秀良 中国科学院海洋研究所山东 青岛 266071
青岛海洋科学与技术试点国家实验室 海洋生物学与生物技术实验室山东 青岛 266237 
林学政 自然资源部第一海洋研究所山东 青岛 266061 
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中文摘要:
      南极红藻具有重要的生态学功能和开发利用价值。南极极端环境赋予了其独特的生理耐受机制,也是发现新基因和代谢途径的理想材料。我们测序分析了南极产胶红藻Iridaea cordata (Turner) Bory和Curdiea racovitzae Hariot的转录组序列,并与其常温近缘种进行了比较,同时挖掘了其与光限制和强紫外线辐射等光环境适应相关的基因。I. cordataC. racovitzae的转录组序列分别拼接成了14055条和12006条非冗余基因,平均长度分别为1473 bp和1448 bp。在I. cordata转录组中发现多条与绿藻基因同源的捕光复合物LHC基因Lhca2Lhca6Lhcb,并且在两种南极红藻中都各发现了1条编码结合岩藻黄质和Chl a/c蛋白的Lhcf基因,目前尚未在其他红藻中发现这类基因。光裂解酶修复紫外线诱导DNA损伤,在I. cordata的转录组序列中发现了6-4光裂解酶,光裂解酶CPD I和CPD II基因,而C. racovitzae转录组序列中仅找到了光裂解酶CPD II基因。尽管南极红藻中这些特有基因的功能有待进一步的验证,但是本文为后续研究红藻的南极极端光环境适应机制提供了方向。
英文摘要:
      Antarctic red algae play important roles in the coastal ecosystems and industrial applications. Meanwhile, their unique physiological acclimation mechanisms to the extreme environments endow them to be ideal organisms for discovering new genes and new metabolic pathways. In this study, we sequenced the transcriptomes of Antarctic red algae Iridaea cordata (Turner) Bory and Curdiea racovitzae Hariot, and compared with their moderate temperature close relatives. The transcriptome sequences of I. cordata and C. racovitzae were assembled into 14055 and 12006 Unigenes, with an average length of 1473 bp and 1448 bp, respectively. The Lhca2, Lhca6 and Lhcb genes homologous to the green algae genes were found in I. cordata transcriptome while not in other red algae. Lhcf gene encoding fucoxanthine and Chl a/c binding protein presenting in brown algae and diatoms were identified in both I. cordata and C. racovitzae. Photolyase repairs UV-induced DNA damages. 6-4 photolyase, CPD I and CPD II genes were identified in the transcriptome of I. cordata, while only CPD II gene was found in the transcriptome of C. racovitzae. Although the functions of those specific genes in Antarctic red algae are expected further investigation, our study provides a foundation for the following researches on the acclimation mechanisms of seaweeds to the extreme light environments in Antarctica.
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