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李淑平,严兴洪.60Co-γ射线辐照对长紫菜的诱变效果及优良品系分离与特性分析[J].海洋学报,2015,37(10):69-79
60Co-γ射线辐照对长紫菜的诱变效果及优良品系分离与特性分析
Isolation and characterization of an improved strain of Pyropia dentata (Bangiales, Rhodophyta) after being irradiated by 60Co-γ ray
投稿时间:2015-04-29  
DOI:10.3969/j.issn.0253-4193.2015.10.007
中文关键词:  长紫菜  叶状体  60Co-γ射线  色素变异  体细胞再生体  优良品系
英文关键词:Pyropia dentata  blades  60Co-γray  pigmentation mutation  regenerated blades  improved strain
基金项目:国家高科技研究发展计划(863计划)资助项目(2012AA10A411);国家自然科学基金资助项目(31072208);农业部公益性专项(200903030);国家农业科技成果转化资金项目(2013GB2C220537)。
作者单位E-mail
李淑平 上海海洋大学 水产与生命学院, 上海 201306  
严兴洪 上海海洋大学 水产与生命学院, 上海 201306
上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306 
xhyan@shou.edu.cn 
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中文摘要:
      长紫菜野生型品系(PD-WT)的叶状体经60Co-γ射线辐照处理后再培养4周,出现了枣红、浅桔红、黄绿、浅桔黄等颜色的色素变异细胞块,其百分率随着辐照剂量的增加而增加;同时,从基部到梢部,其变异率也呈现上升趋势。利用酶解法获得的单个色素变异细胞经离体培养后再生成叶状体,从再生体中筛选出具有明显生长优势的新品系(PD-5)。培养30~70 d,PD-5 品系的叶状体最大绝对生长率和平均绝对生长率分别为3.76 cm/d和2.71 cm/d,分别是PD-WT品系的3.60倍和4.22倍。日龄70 d时,PD- 5 品系的叶状体平均体长为117.42 cm,是PD-WT品系的4.32倍。日龄45 d的叶状体,PD-5 品系的叶绿素a和总藻胆蛋白含量分别为8.41 mg/g和97.07 mg/g,分别比PD-WT品系增加了25.71%和104.44%;PD-5 品系的叶状体平均厚度为26.79 μm,比PD-WT品系降低了35.04%。PD- 5 品系的壳孢子放散总量高达421.16 万个/贝壳,是PD-WT品系的2.19倍。综上所述,与PD-WT品系相比,PD-5品系在叶状体的生长、3种主要光合色素和色素蛋白含量以及壳孢子放散量等方面,均表现出很明显的优势,有望被培养成可大规模栽培的新品种。
英文摘要:
      The young gametophytic blades of Pyropia dentata, developed from conchospores of the wild-type strain (PD-WT), were treated with 60Co-γ ray to induce mutation in the experiment. After being cultured for 4 weeks, there were many color-mutated cell clusters showing bright red, red brown, red orange and yellow green in the treated blades. The results showed that the percentage of the colored-mutated cell-clusters increased as increasing of the irradiation dose and from the base to the tip of the blades. Single color-mutated cells were isolated enzymatically from the color-mutated blades and were regenerated into blades. An improved strain named as PD-5 strain with the growth advantages was selected from the regenerated blades. In the growth of the blades, the maximum and average absolute growth rates of this improved strain were 3.76 cm/d and 2.71 cm/d, which were 3.60 and 4.22 times that of the PD-WT strain, respectively, during culture from 30 to 70 days. The mean length of F1 gametophytic blades of PD-5 strain was 117.42 cm which was 4.32 times that of the PD-WT strain after being cultured for 70 days. The contents of Chl a and phycobiliprotein of thePD-5 strain were 8.41 mg/g and 97.07 mg/g, respectively, increasing by 25.71% and 104.44% in contrast with that of the PD-WT,respectively. The mean thickness of the 45-day-old blades of the PD-5 was 26.79 μm,decreasing by 35.04% in contrast with that of the PD-WT. The total numbers of the conchospores released from the PD- 5 strain was 421.16×104 per shell,which was 2.19 times that of the PD-WT strain. The aboved results confirmed that the PD-5 strain was characterized by faster growth,higher contents of photosynthetic pigments,larger amount of releasing conchospores than the wild-type strain. Therefore,the improved strain PD-5 had great potential to be applied in commercial cultivation as a new strain.
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