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南海赤潮亚历山大藻的分子鉴定及“塔玛复合种”的分子系统发生分析
Molecular identification of red tide Alexandrium isolated from the South China Sea and phylogenetic analysis on “tarmarensis complex"
投稿时间:2009-11-19  修订日期:2010-05-26
DOI:
中文关键词:  “塔玛复合种”  SSU rDNA  ITS  分子系统发生
英文关键词:"Tarmarensis complexe"  SSU rDNA  ITS  Phylogenetic
基金项目:国家海洋局青年基金项目(2008110)
作者单位邮编
刘晨临* 国家海洋局第一海洋研究所 266061
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中文摘要:
      在本文中我们利用SSU rDNA和ITS分子指标,结合GenBank和其它文献中的基因序列,构建了系统树,对一株分离自我国南海的亚历山大藻“塔玛复合种”NH01进行了鉴定,发现SSU rDNA 和ITS 序列构建的系统树中,不同地理基因型的塔玛复合种均构成独立分支。与所有分析的中国沿海“塔玛复合种”一致,NH01也属于“亚洲温带”型。文中探讨了SSU rDNA和ITS分子指标用于塔玛和链状亚历山大藻鉴别的可行性,比较了2种不同的基于ITS序列的亚历山大藻的系统分类方法。认为SSU rDNA和ITS序列均可以应用于亚历山大藻种甚至种下水平的检测,可以准确判断其地理基因型。同时提出利用SSU rDNA和ITS等核酸序列标记的方法,作为鉴定、调研我国海域的亚历山大藻“塔玛复合种”的常规手段。另外,本研究通过比对不同地理基因型的ITS序列,确定了一段DNA针序列,可以用于鉴别不同地理基因型的亚历山大藻“塔玛复合种”。
英文摘要:
      In this paper, to identify an Alexandrium "tarmarensis complexes" NH01 isolated from the South China Sea, phylogenetic trees were constructed with SSU rDNA and ITS sequences combined with that from GenBank and literatures. In both SSU rDNA and ITS phylogenetic tree, "tarmarensis complexes" of different geographical genotypes were subdivided into independent clades. Consistent with other "tarmarensis complexes" isolated from China coast area, NH01 also are "temperate Asia" type. The feasibility of identification of "tarmarensis complexes" with SSU rDNA and ITS sequences was conducted, and two kinds of system classification methods based on ITS sequence were discussed. We thought that SSU rDNA and ITS sequences can be used as molecular markers to identification Alexandrium between or even within species, can accurately determine their geographic genotype. We suggested making it a routine method of using SSU rDNA and ITS nucleotide sequence tag approach, as the identification, investigation Alexandrium of our coast. Meanwhile, in this study, a DNA probe based on ITS sequences was identified and can be used to identify different geographical genotypes of "tarmarensis complexe"
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