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中国沿海6个地理群体短蛸的遗传变异研究
Genetic variation in different populations of Octopus ocellatus in China coastal water based on the COI gene analysis
投稿时间:2009-04-08  修订日期:2009-10-19
DOI:
中文关键词:  短蛸  遗传变异
英文关键词:Octopus ocellatus  Genetic variation
基金项目:国家科技攻关计划
作者单位邮编
吕振明* 浙江海洋学院 316000
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中文摘要:
      海洋生物种群遗传变异的研究是实现其资源长期有效管理的基础。本文采用线粒体COI基因序列测定技术分析了我国沿海重要经济头足类短蛸(Octopus ocellatus)6个地理群体的遗传结构及其变异,结果表明我国短蛸资源存在相对丰富的遗传多样性,654bp长度的核苷酸片段共检测到42个多态位点,多态位点比例达6.42%,检测的60个个体中共获23个单倍型,单倍型多样性指数(H)达0.200-0.867,平均核苷酸多样性指数(Pi)达0.0003-0.0097,平均核苷酸差异数达(K)0. 200-6.311,高于多数海洋头足类的遗传变异。对6个群体的遗传结构进行检测表明,我国的短蛸群体间除部分群体外均存在显著的遗传分化(p﹤0.05),遗传结构基本符合脚踏石模型。聚类分析表明6群体已明显分化为两支,一支由是大连、青岛、连云港群体组成,另一支由舟山、厦门和广州群体组成,两类群间存在20个固定位点核苷酸差异,编码的蛋白质存在2个固定氨基酸残基的差异。两类群的分化系数Fst达0.8602(p﹤0.01),基因流大大小于1;AMOVA检测显示仅有12.72%的遗传差异存在于群体内部,而87.28%的遗传差异存在于群体间。本研究成果可为今后我国短蛸资源更好的开发和管理提供理论基础。
英文摘要:
      Abstract: Studies on genetic variation in marine organisms are an important component of successful and sustainable management of marine biology resources. In this paper, genetic variatiaon of 6 populations of Octopus ocellatus in China coastal water were investigated by applying mitoch -ondrial gene(COI) sequencing technology. Results showed that ,Compared with other cephalopod species, Octopus ocellatus showed higher genetic diversity in populations. 42 polymorphic sites were detected in 654bp aligned sequence, which reprented for 6.42% of the total length of sequenced COI gene. 23 hapotypes were detected out of 60 individuals from 6 populations. The hapotype diversity (HD) , mean nucleotide diversity(pi) and average number of nucleotide differences reached 0.200-0.867, 0.0003-0.0097, 0.200-6.311, respectively. Molecular variance analysis revealed strong genetc sturcture in 6 Octopus ocellatus populations(p﹤0.05). A pattern of genetic-isolation-by-distance, comforming to Stepping Stone Model, was detected when plotting Nm against geographic distance.UPGMA tree constructed showed that 6 Octopus ocellatus populations can be divided into two lineages, one consisiting of Dalian, Qingdao,Lianyungang plulation and the other consisting of other 3 populations, and between which, 20 fixed nucleotide sites and 2 fixed amino acid sites subtitute along the gene sequence and coded protein were detected. The Fst between them reached 0.8602 (p﹤0.01) and the Nm between them turned out to be much lower than 1. AMOVA test, which showing 12.72% of the genetic variation lies within populations and 87.28% lies between populations, further suggested the genetic differentiation between the two lineages. These findings in the aspect of genetic variation of Octopus ocellatus will contribute to better exploitment and refined management of this precious cephalopod resources along the coast of China in future.
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